Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide.
نویسندگان
چکیده
A purification procedure for fish myosin heavy chain is described. The protein was injected into rabbits for production of antibodies. The specificity of the antibodies was determined by immunoblotting. The enzyme-linked immunosorbent assay technique was applied to quantify myosin heavy chain bound to isolated polyribosomes of epaxial muscle from fish.
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عنوان ژورنال:
- The Biochemical journal
دوره 232 2 شماره
صفحات -
تاریخ انتشار 1985